Junsang Doh, Ph.D.

Professor
I-Bio / Department of Mechanical Engineering
Immuno-MEMS and Cellular Dynamics

bar04_solid1x1_gray.gif

E-mail jsdoh@postech.ac.kr
Phone +82-54-279-2189 (office)
          +82-54-279-5555 (lab.)
Laboratory Immuno-MEMS and Cellular Dynamics

bar04_solid1x1_black.gif

Profile |  Research Interests  |  Selected Publications

bar04_solid1x1_black.gif

           

Profile

2006
2006

2006-2008

Ph.D., Massachusetts Institute of Technology
Postdoctoral Fellow, Department of Materials Science and Engineering, Massachusetts Institute of Technology
Postdoctoral Fellow, Department of Pathology, University of California at San Francisco

 

black01_up.gifTOP

     

Research Interests

We are developing and applying interdisciplinary approaches for the study of T cell biology. Our lab is composed of members with extremely diverse undergraduate backgrounds including polymer science, life science, mechanical engineering, biomedical engineering, and veterinary medicine.

T cells orchestrate antigen-specific immune responses, thus understanding the biology of T cell is essential for the development of therapeutics against various immune related diseases such as rheumatoid arthritis, asthma, and cancers.

T cells are extremely versatile cells adjusting themselves to various physiological/ pathological microenvironments to successfully mount antigen-specific immune responses. How T cell adapt themselves under such diverse microenvironments are poorly understood so far mostly due to technical limitations. We are addressing this challenging problems by developing and applying state-of-an-art technology developed in physical science/engineering.

Firstly, we are developing microfabricated devices to mimic complex microenvironments T cells navigating and dissect complex cell-cell communication.

Secondly, we are applying state-of-an-art imaging techniques for the study of molecular/cellular dynamics. Two-photon microscopy, in collaboration with Prof. Ki-Heon Kim, is used to observe stunning cell dynamics in intact organs. Total internal reflection fluorescence microscopy (TIRF), in collaboration with Prof. Wonhwa Cho, is used to image single molecule dynamics of molecules related with the immunological synapses in T cells.

Thirdly, we are developing biomechanical approaches to correlate mechanical microenvironments with immune responses. Biochemical cues important for immune responses have been extensively studied, but biomechanical cues have not been paid any attention up to date.

Together, these new tools will open new opportunities to better understand immune system and provide new knowledge important for the diagnosis and treatment of immune-related diseases.

 

      

Selected Publications 

  • M. Kim, J.-C. Choi, H.-R. Jung, J.S. Katz, M.-G. Kim, and J. Doh*, Addressable micropatterning of multiple proteins and cells by microscope projection photolithography based on a protein friendly photoresist, Langmuir (in press).
  • J. Doh*, M. Kim and M.F Krummel*, Cell-Laden Microwells For The Study Of Multicellularity In Lymphocyte Fate Decisions, Biomaterials. 31 , 3422 (2010). *Co-corresponding authors.
  • J. Doh and M.F. Krummel, Immunological synapses within context: patterns of cell-cell communication and their application in T-T interactions, Curr. Topics in Microbiol. Immunol. 340, 25 (2010).
  • C.A. Sabatos#, J. Doh# , S. Chakravarti, P.G. Pandurangi, R.S. Friedman, A.J. Tooley and M.F. Krummel, A Synaptic Basis for Paracrine Interleukin-2 Signaling in Activating T cells. Immunity   29 , 238 (2008) #Equal contribution
  • J. Doh and D.J. Irvine, Immunological synapse arrays: Patterned protein surfaces that modulate immunological synapse structure formation in T cells, Proc. Natl. Acad. Sci. USA 103 (15), 5700 (2006).
  • J. Doh and D.J. Irvine, Photogenerated polyelectrolyte bilayers from an aqueous-processible photoresist for multicomponent protein patterning., J. Am. Chem. Soc. 126 (30), 170 (2004).

 

 

bar04_solid1x1_gray.gif